Oligonucleotides



Oligo Synthesis

Sentegen carries out high quality primer synthesis using the world’s most advanced high-throughput DNA synthesis and purification systems. Our high-throughput system is able to meet the annual oligo needs of Turkey. Sentegen offers quality and pricing that is competitive with other oligo synthesis companies. With fast same-day delivery and low prices, we provide our high quality primers to researchers and clinicians.

With our lab’s newly acquired Biolytic (USA) Dr. Oligo™ DNA/RNA Synthesizer, 96 oligos can be simultaneously synthesized separately with a yield of >99.95%. Using Dr. Oligo™, our lab routinely performs synthesis of oligos up to 125 bp in length at standard 25 nmol, 100 nmol and 200 nmol scales.

Furthermore, using the fully automated Dr. Oligo™Processor, we carry out routine purification of our synthesized oligos including removal of organic salts (desalting) and DMT-ON (Oligonucleotide Purification Column (OPC)-Reverse Phase (RP)-HPLC grade). If desired, we also offer PAGE and Ion Exchange (IE)-HPLC grade purification.

senteligo™

We offer senteligo™ brand RP-HPLC grade quality primer products at 200 nmol scale. senteligo™ primers are RP-HPLC grade purified using fully automated OPC purification systems.

senteligo™ advantages:
  • High quality
  • OPC (RP-HPLC grade) purification
  • Competitive prices
  • Fast delivery (delivery within 24 hours)
  • Guaranteed

Synthesis, Delivery and Storage Conditions

Storage Conditions Temperature Maximum Storage Duration
Lyophilized -200C < 1 year
Lyophilized Room temperature ≤ 1 month
Re-suspended -200C < 6 months
Re-suspended Room temperature ≤ 1 week

Purification options include standard purification, OPC (RP-HPLC grade), PAGE and IO-HPLC.

Standard desalting (removal of small molecule impurities) is available for every oligonucleotide. If desired, orders within Ankara can be delivered via cold chain as 100 uM normalized stock concentration.

Prior to drying, the OD and A260 values are calculated and the necessary amount of TE or H2O to be added to produce a stock solution is determined. This information is given to the customer as a report at the time of product delivery.

Lyophilized primers can be stored at room temperature. Re-suspended primers must be stored at -20C. For long-term storage of your primers, dissolve with TE Buffer and avoid freeze-thaw cycles. Dilute the amount to be used and store this as aliquots.

Terms of Guarantee

Sentegen guarantees your primers. The primers are guaranteed for 3 months. If desired, primers can be re-synthesized within the 3-month guarantee period. Primers less than 15 bp or above 40 bp are not included in the guarantee. Primers with mistakes in the sequence that you provide or primers that are not stored after delivery according to the storage conditions are also not covered by the guarantee.

Additional conditions for the guarantee to hold:
  • Primer sequence must previously have been shown to work.
  • Primer sequence must be published in an international journal.

Quality Control


The synthesized primers are routinely checked by gel electrophoresis and PAGE to ensure only a single band was produced. Futhermore, we also perform quality control by PCR and gene synthesis experiments. In each oligo production, during the many deblock steps that are carried out, the production data is checked by trityl analysis. The synthesis results are presented as a report with the OD 260 value as measured using the NanoDrop spectrophotometer. Monthly LC-MS analysis ensures continued quality assurance.

Price List

  • Our synthesis days are Monday and Wednesday.
  • Orders received by 16:00 on Monday and Wednesday for oligos less than 40 bases long and at 100 nmol scale will be ready the following day at 16:00.
  • Orders are manufactured in the order in which they are received. If the synthesis capacity is filled that day, your order will be added to the next synthesis.
  • Orders for 40 or more oligos are considered bulk orders. For delivery time, please contact us.25 nmol, 200 nmol, OPC içeren siparişler ile 40 baz ve üzeri oligolar Pazartesi ve Çarşamba dışındaki günlerde sentezlenir. Teslimat süresi için bize ulaşınız.
  • Oligonucleotides of 25 nmol, 200 nmol, OPC and greater than 40 bases are synthesized on other days. For delivery time, please contact us.

Price List (Updated on 11.01.2016)

To submit an order, please fill in the form and send by email to: order@sentegen.com

Order Form


Scale of Synthesis Price (€) Standard Purification (Desalting) OPC Purification (RP-HPLC Grade) PAGE Purification IE-HPLC Purification
  Per Base Up to 40 bases Per Primer Up to 60 bases Per Primer Up to 100 bases Per Primer Up to 120 bases
25 nmol (min 2 OD) 0,29 € Free 10 € 15 € 30 €
25 nmol (min 2 OD) 0,49 € Free 10 € 15 € 30 €
200 nmol (min 12 OD) 0,59 € Free 10 € 15 € 30 €
senteligo™ 200 nmol(min 6 OD) 0,89 €* - Free - -
* 24-hour delivery is applicable to 100 nmol synthesis. Delivery is free for orders of more than 12 primer pairs. Shipping price is 8 TL. 18% tax is not included. Contact us for special pricing on annual and bulk orders. Tel: 0312 265 06 62

Scale and Purification Selection Guide – by Application

  Desalted OPC (RP-HPLC Grade) PAGE IE-HPLC
  Up to 40 bases Up to 60 bases Up to 100 bases Up to 150 bases
25 nmol PCR - - -
100 nmol PCR, RT-PCR PCR, Sequencing, RT-PCR, Next Generation Sequencing, Microarray - -
200 nmol PCR, RT-PCR PCR, Sequencing, RT-PCR, Next Generation Sequencing, Microarray PCR, Sequencing, RT-PCR, Next Generation Sequencing, Microarray Sequencing ,RT-PCR, Aptamer
senteligo™ - PCR, Sequencing, RT-PCR, Next Generation Sequencing, Microarray - -

Coupon pricing

  Coupon 1000 Coupon 2500 Coupon 5000 Coupon 10000
25 nmol - 0,26 € 0,23 € 0,19 €
100 nmol 0,39 € 0,36 € 0,33 € 0,29 €
200 nmol 0,49 € 0,46 € 0,43 € 0,39 €
Coupon 1000/2500/5000/10000 - Order 1000/2500/5000/10000 bases respectively within one year with advance payment.

General Information

Oligonucleotides are short strands of DNA. Oligonucleotides (primers) are used in many molecular and genetic manipulations such as PCR. They are also used to determine base sequences in a DNA strand and identify mutations in DNA.

Sentegen synthesizes cost-effective nucleotide sequences for you that can be used in molecular biology studies. The primers we produce undergo quality control testing. The oligonucleotide synthesis process is based on tetrazole catalysis with phosphoramadite monomers.

Phoshporamidite is a normal nucleotide except that it contains a protecting group such as trityl. These groups block reactive amine, hydroxyl and phosphate groups. The protecting groups prevent undesired side reactions and ensure production of the desired product. After the synthesis process is complete, these protecting groups are removed.

The first phosphoramidite molecule attaches to a solid surface from the 3’ end and synthesis progresses in the 3’ to 5’ direction. The solid surface that the protected nucleotides bind is a 5-micrometer glass bead that contains pores and canals of controlled size for the initial nucleotide to adhere.

Phorphoramide based synthesis is carried out via the serial repitition of 4-step cycles and is completed once the next nucleotide is bound to the 5’ terminus. These 4 steps are: deprotection, coupling, capping and stabilization, respectively.


Primer purification methods:

This section is designed to help you choose the purification method most suited for your application. During oligo synthesis, every nucleotide is added to the growing chain successively. In each assembly cycle, nucleotides are unable to be added to a small fraction of the oligo chains. This results in the production of a mixture of both full-length and short sequences. During oligo synthesis, the separation of support and removal of protecting groups results in residues. These residues are removed by purification.

For some applications, it is very important that only full-length (n) oligos are present. For other applications, the presence of shorter oligos (n-1, n-2, etc.) does not affect experimental results.

Desalting
Oligonucleotides produced by Sentegen are purified by the desalting method. The desalting process is used to clean up side products and residues that are produced during synthesis. This method is useful for oligos up to 35 bp and PCR applications.
PAGE
The PAGE separation technique is based on relative charge/molecular weight. With this method, separation by length can be carried out. This results in a purity of 95-99%. The yield of the PAGE method is less than that of other purification methods. In this method, gel extraction must be used to separate the oligos of desired length from shorter oligos and residues. This method is recommended for experiments in which high purity oligonucleotides are required. The PAGE method is recommended for oligos greater than 50 bases in length.
OPC
With the OPC method, incorrect sequences, side products and impurities are removed. With this method, the primers obtained can be used for sequencing, PCR, hybridization probes and gene synthesis. OPC purification is recommended for oligonucleotides up to 70 bases in length.
HPLC Reverse-phase

This separation technique is based on the difference in hydrophobicity between the products of the desired length (containing the 5’-DMT group) and short sequences (without the DMT groups). The DMT oligo of desired length is bound to a column while the short sequences are washed away. Subsequently, with the DMT fragmentation method, the desired product is acquired.

Purification of oligos using fluorophores is a productive method. The lypophilic characteristic of fluorophores creates an excellent environment for the removal of impurities after synthesis. Futhermore, RP-HPLC is a preferred method for large scale syntheses. Since the lipophilic-based separation will result in shortened oligos, this method is not recommended for oligos less than 50 bases long.