DNA Probe


DNA Probe

DNA probes are used in in-situ techniques to determine whether or not a target sequence is present by hybridization with the target DNA or RNA sequence. The markers found on these hybridized probes are what allow them to be visualized. In medicine, this method is used to identify microogranisms and diagnose infectious, genetic and other diseases. Sentegen synthesizes molecular probes that you can use in your molecular biology research. The manufactured primer sequences are subjected to quality control tests. We are able to modify probes with the following markers: biotin, FAM, 6-Hexachloro Fluorescein, BHQ-1 and phosphate.

Our probe synthesis services will begin in December 2015.


Probe purification methods:
  • This section is designed to help you choose the purification method most suited for your application.
  • During oligo synthesis, every nucleotide is added to the growing chain successively.
  • In each assembly cycle, nucleotides are unable to be added to a small fraction of the oligo chains. This results in the production of a mixture of both full-length and short sequences.
  • During oligo synthesis, the separation of support and removal of protecting groups results in residues. These residues are removed by purification.
  • For some applications, it is very important that only full-length (n) oligos are present. For other applications, the presence of shorter oligos (n-1, n-2, etc.) does not affect experimental results.