Sequencing Services



Sequencing Services

Sentegen Biotechnology sequencing services are available for all your sequencing needs. Using ABI Prism Genetic Analyzer, PGM Ion Torrent and Illumina MiSeq devices we can sequence your genetic material by either conventional Sanger sequencing or Next Generation Sequencing and deliver your results electronically in a short time. We guarantee up to 600bp in Sanger Sequencing and we can sequence up to 20KB of genetic material via NGS.


Sanger Sequencing

With Sanger Sequencing, gel or column-purified PCR products and plasmids can be used as starting material. Sequencing results can typically be obtained for up to 600 nucleotides. The resulting output of sequencing depends on the purity and quality of the starting material. If desired, we can deisgn and synthesize suitable primers. With paired-end sequencing it is possible to obtain more accurate results. In our lab, the ABI Prism 3130 Genetic Analyzer is used for sequencing services.

The working principle of the machine is the generation of a monochromatic light via a laser located within the electrophoretic units. The gel-matrix that the DNA is loaded into is scanned by the monochromatic light. In the electrophoresis step, when the region with the fluorescent dye-bound DNA is arrived at, a signal is given and a characteristic wavelength of light is scattered. This signal is received by the detector and the results obtained are assessed and then turned into sequence data.




Price Table
  Price (€) Bulk order of 500 Bulk order of 1000
Sanger Sequencing of Plasmids 6,99 € 5,99 € 4,99 €
Sanger Sequencing of PCR Products 6,99 € 5,99 € 4,99 €
NGS Sequencing of Plasmids (max: 20 kb) 19,99 €    
NGS Sequencing of PCR products (max: 20 kb) 19,99 €    
PCR Product Purification 1 €    
  • NGS services are provided using PGM Ion Torrent™ ve Illumina MiSeq™.
  • Other NGS services (RNASeq, WGS, etc.) are undertaken as independent projects. Please contact us for details.

Required Information for Analysis of DNA Sequences.
  • DNA samples and primers (sequencing order form should be filled out and sent to us).
  • Concentration of primers to be used in sequencing (at least 10 uM concentration and at least 5 uL of primer/reaction) and sequence information (preferred).
  • Concentration of DNA sample (for plasmids, at least 80 ng/uL and 5-10 uL/reaction is recommended; for PCR products, at least 10 ng/uL and 5-10 uL/reaction is recommended)
  • Gel image and spectrophotometric measurement results (concentration, A260/280 and A260/230 values) for PCR products (run in 2% agarose gel) or plasmid, phage, cosmid or BAC DNA (run in 1% agarose gel).

Requirements for DNA Sequence sample delivery

Requirements for sample preparation
  • Sentegen accepts both purified and unpurified PCR products and plasmid DNA
  • To ensure optimal concentration, we recommend the values shown in the table below
  • To ensure that there is only one band in purified PCR products, they should be checked by gel electrophoresis.
  • Samples must be clearly labeled.
Recommended sample values
Sample Concentration Volume / RXN
Plasmid 200 ng/μl 10μl
PCR products 50 ng/μl 10μl
Primer 10μM 10μl
Sample Transfer Requirements:
  • All of your samples, including the primers, should be provided in 1.5 mL, 0.5 mL or 0.2 mL tubes and should be labeled in the manner in which you wish the sample to be identified.
  • Sentegen is not responsible for problems such as leakage, evaporation or cross-well contamination (due to the samples not being prepared in the proper manner) or problems arising during delivery.

Some Common primers used at Sentegen
Primer Name Sequence
5'AD 5'- AGG GAT GTT TAA TAC CAC TAC -3'
3'AD 5'- AGA TGG TGC ACG ATG CAC AG -3'
5AOX1 5'- GAC TGG TTC CAA TTG ACA AGC -3'
3AOX1 5'- GCA AAT GGC ATT CTG ACA TCC -3'
A-FACTOR 5′-TAC TAT TGC CAG CAT TGC TGC -3'
3'BD 5'- TAA GAG TCA CTT TAA AAT TTG TAT C -3'
CMV-Forward 5'- CGC AAA TGG GCG GTA GGC GTG -3'
CMV-R 5'- GTT CAC GGT GCC CTC C -3'
PGEX-5' 5'- GGG CTG GCA AGC CAC GTT TGG TG -3'
PGEX-3' 5'- CCG GGA GCT GCA TGT GTC AGA GG -3'
T3 5'- ATT AAC CCT CAC TAA AGG GA -3'
T7 5'- TAA TAC GAC TCA CTA TAG GG -3'
T7-Ter 5'- GCT AGT TAT TGC TCA GCG G -3'
BGH 5'- TAG AAG GCA CAG TCG AGG -3'
SP6 5'- GAT TTA GGT GAC ACT ATA G -3'
M13F 5'- TGT AAA ACG ACG GCC AGT -3'
M13R 5'- CAG GAA ACA GCT ATG AC -3'
M13F(-47) 5'- CGC CAG GGT TTT CCC AGT CAC GAC -3'
M13R(-48) 5'- AGC GGA TAA CAA TTT CAC ACA GGA -3'
GLP1 5'- TGT ATC TTA TGG TAC TGT AAC TG -3'
GLP2 5'- CTT TAT GTT TTT GGC GTC TTC CA -3'
pBAD Forward 5'- ATG CCA TAG CAT TTT TAT CC -3'
pBAD Reverse 5'- GAT TTA ATC TGT ATC AGG -3'
pEGFP-N-5' 5'- TGG GAG GTC TAT ATA AGC AGA G -3'
pEGFP-N-3' 5'- CGT CGC CGT CCA GCT CGA CCA G -3'
pEGFP-C-5' 5'- CAT GGT CCT GCT GGA GTT CGT G -3'
pEGFP-C-3' 5'- TAT GGC TGA TTA TGA TCA GT -3'
pFastBacF 5'- GGA TTA TTC ATA CCG TCC CA -3'
pFastBacR 5'- CAA ATG TGG TAT GGC TGA TT -3'
RVP3 5'- CTA GCA AAA TAG GCT GTC CC -3'
RVP4 5'- GAC GAT AGT CAT GCC CCG CG -3'
PMAL-C2X-F 5'- TGC GTA CTG CGG TGA TCA AC -3'
PMAL-C2X-R 5'- CTG CAA GGC GAT TAA GTT GG -3'
PQE30F 5′- TGA GCG GAT AAC AAT TTC AC-3'
PQE30R 5′- GTT CTG AGG TCA TTA CTG G-3'
S.TAG 5'-CGA ACG CCA GCA CAT GGA CA-3'

Next Generation Sequencing

Our lab provides the following services using Ion Torrent PGM and MiSeq next generation sequencing platforms:


Amplicon Sequencing
Targeted Sequencing
Targeted sequencing is a quick and cost-effective alternative for whole genome sequencing. AmpliSeq™ technology allows researchers to quickly and easily amplify thousands of targets from just 10 ng of sample DNA.
Small RNA and miRNA Sequencing
Cutting edge Ambion® RNA technology combined with Ion Torrent™ next generation sequencing give labs more research and discovery opportunities.
Virus Typing
Using the Ion PGM system’s PathAmp™ FluA kit together with Pathogen Analyzer Plugin for Torrent Suite™
Microbial Sequencing
With the Ion PGM system’s capabilities of increased data output, high accuracy and long reads, retrospective analysis of specific disease types, surveillance, outbreak and disease etiology is possible.
De Novo Sequencing
Ion Torrent semiconductor sequencing offers quick and low-cost de novo sequencing for microbial research.
Bacterial Typing
The ION PGMTM system enables typing of bacterial species with high accuracy and precision by using sequence data from hundreds of genes.