Next Generation Sequencing

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Next Generation Sequencing

Sentegen utilizes the Ion Torrent Personal Genome Machine (PGM) to provide next generation sequencing services. The PGM sequencer is a bench-top system that utilizes semi-conductor technology. It enables rapid and scalable sequencing. Ion Torrent Technology uses a large number of parallel semiconductor sensors to quantitate (in real-time) hydrogen ions released during DNA replication.

Compactly organized arrays of wells serve as reactors for millions of parallel reactions. Integrated fluidics enable the flow of reagents from these wells into the array of sensors. These unique fluidics, with the help of micromachinery and semi-conductor technology, translates genetic sequence into digital data and can have an output of up to 2000 Mbs (318 chips). An average sequencing process takes about 2-3 hours.